200 TECHNIC, 



(7) Remove excess of stain with thirty-five per cent 

 alcohol. 



(8) Dehydrate with alcohol. 



(9) Clear up with creosote, five to ten minutes. 



(10) Wipe off excess of creosote and mount with 

 balsam. 



(u) Center cover-glass. 



(12) Label and keep in horizontal position until 

 balsam hardens. 



NO. 7. HAEMATOXYLIN METHOD FOR AFFIXED 

 DIN SECTIONS. 



( I ) Center section and affix with collodion mixture. 



(2) Stain with diluted hsematoxylin, one to ten min- 

 utes. 



(3) Remove excess of stain with acid alcohol. 



(4) Apply seventy per cent alcohol. 



(5) Apply eighty per cent alcohol. 



(6) Apply ninety-five per cent alcohol a few sec- 

 onds. 



(7) Clear up with creosote, five to ten minutes. 



(8) Remove excess of creosote with blotting paper. 



(9) Mount with balsam and center cover-glass. 



