REAGENTS AND STAINS. 201 



(10) Label and lay aside in a horizontal position 



until balsam is hardened. 



/ 



NO. 8. HAMATOXYUN-OSIN METHOD. 



(1) If desired, affix section to slide with egg-albu- 

 men and glycerine or collodion and clove-oil. 



(2) If a paraffin -section, remove paraffin with xylol 

 or turpentine or both ; remove xylol, etc., with alcohol. 



(3) Apply thirty-five per cent alcohol. 



(4) Stain with diluted hsematoxylin, one to five 

 minutes. 



(5) Apply thirty-five per cent alcohol to remove ex- 

 cess of stain. 



(6) Stain with alcoholic eosin about five minutes. 



(7) Apply ninety-five per cent alcohol to remove ex- 

 cess of stain and dehydrate. 



(8) Clear up with cresote. 



(9) Remove excess of creosote and mount with bal- 

 sam. 



(10) Center cover-glass and label. 



(n) Lay aside in horizontal position until balsam 

 hardens. 



Staining Unicellular Organisms. 

 It is often desirable to examine materials \\ithout 

 staining. This is accomplished by placing upon the 



