202 TECHNIC, 



glass slip a drop of the material tc be examined and 

 applying cover-glass. A hair placed under the cover 

 glass will prevent the object from being crushed and 

 allow of free motion in the case of living organisms. 

 Should it be desired to stain such preparations, two 

 methods may be pursued, irrigation and cover-glass 

 staining. 



NO. 9. IRRIGATION AND STAINING MICRO-ORGANISMS. 



(1) Place upon the slide a drop of material to be 

 studied. 



(2) Apply cover-glass. 



(3) At the edge of the cover-glass, by means of a 

 pipette, place a drop or two of the reagents or stain. 



(4) By means of a triangular piece of blotting paper 

 applied at the opposite edge of the cover-glass, absorb 

 the moisture from the preparation, thus drawing under 

 the stain. 



NO. 10. COVR-GI,ASS PREPARATIONS. 



(1) Make a thin spread of the substance to be ex- 

 amined upon a sterilized cover-glass. 



(2) Using a Cornet forceps, dry the preparation by 

 holding it between the fingers above the flame. 



