206 TECHNIC, 



bones, serving at the same time as a staining reagent. 

 Crystals should be added from time to time, so that 

 some undissolved crystals will always remain in the 

 bottom of the vessels. 



Nitric acid. Use a ten per cent volumetric solution 

 in water. Decalcifkation occurs in five to ten days. 



FIXING REAGENTS. 



Absolute alcohol. Specimens should remain in this 

 reagent from one to six hours, according to size. 



Perenyi's fluid 



Nitric acid (ten per cent) 40 cc. 



Chromic acid (0.5 per cent) 30 cc. 



Alcohol 30 cc. 



A good reagent for embryos and adult tissues. Time, 

 three to twelve hours ; dehydrate with alcohol. 



Erlicki's fluid- 



Potassium bi-chromate 2.5 grams. 



Cupric sulphate 0.5 grams. 



Water 100 cc. 



Aqueous Solution 



Corrosive Sublimate 



Corrosive sublimate I gram. 



Water 95 cc. 



