210 TECHNIC, 



Note. This is a valuable stain for vegetable sec- 

 tions. It should be diluted for use as desired. 



Lithium carmine 



Carmine 2.5 grams. 



Lithium carbonate (saturated 



solution) 100 cc. 



The carmine should be dissolved in cold solution. 

 Sections stain rapidly, and. should be decolorized with 

 acid alcohol. 



D ala-field's haeinatoxylin 



1. Hsematoxylin I gram. 



2. Absolute alcohol 6 cc. 



3. Amonium alum (saturated 



sol.) 6 cc. 



4. Glycerine 25 cc. 



Methyl alcohol . . ; 25 cc. 



Process. Dissolve (i) in (2); add this solution to 



(3) ; expose to air and light for a week; filter and add 



(4) and (5) ; allow it to stand for a long time exposed 

 to air and light. 



Alcoholic Eosin for Sections. Make a saturated al- 

 coholic solution. This is used as a ground stain in 

 connection with hsematoxylin ; also as a blood stain. 



