MICROSCOPIC TECHNIQUE. 



I. REAGENTS AND STAINS. 



(l) DECOLORIZING SOLUTIONS. 



Twenty-five per cent aqueous solutions of hydro- 

 chloric, nitric, and sulphuric acids may be used for de- 

 colorizing. 



(2) ACID ALCOHOL. 



Hydrochloric acid I part 



Alcohol (seventy per cent) 100 parts 



(3) IODINE SOLUTION. 



Iodine I gram 



Potassium iodide 2 grams 



Water 90 cc. 



(4) CARBOL tfUCHSIN. 



Fuchsin I cc. 



Alcohol 10 cc. 



Dissolve and add 100 c.c. of five per cent solution of 

 carbolic acid. Filter. 



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