214 TECHNIC, 



(5) ACID METHYLENE BLUE. 



Sulphuric acid 16 cc. 



Water 90 cc. 



Methylene blue 2 grams 



This stain should be prepared fresh from time to 

 time. The carbol fuchsin improves with age. 



(6) LOFFLER'S ALKALINE METHYLENE BLUE. 



Concentrated alcoholic solution of 

 methylene blue 30 c. 



Potassium hydrate (aqueous solu- 

 tion i-ioooo) : 100 cc. 



This is especially useful in staining the bacillus of 

 diptheria. 



(7) ANILINE-WATER GENTIAN VIOLET. 



Aniline oil 5 cc. 



Water 100 cc. 



Mix, shake vigorously, filter; the fluid after filtra- 

 tion should be perfectly clear; add 



Alcohol 10 cc. 



Alcoholic solution of gentian violet, n cc. 



This solution should be freshly prepared about every 

 two weeks. 



