REAGENTS AND STAINS. 217 



(f) Counter- stain with aqueous methylene blue half 

 a minute. 



(g), Wash in water, . 



(h) Dry and clear up with xylol. 



(i) Mount in balsam. 



STAINING, OF 



(a) Mix upon the cover-glass a portion of the cul- 

 ture with a drop of water, using care not to break off 

 the delicate flagella. 



(b) Dry and pass three times through a flame. 



(c) Apply Loffler's mordant one minute, warming 

 gently. 



(d) Wash in water. 



(e) Stain with aniline-water fuchsin. 



(f ) Wash- in water. , 



(g) Dry and mount in balsam. 



GRAM'S METHOD FOR BACTERIA. 



(a) Make a cover-glass preparation by the usual 

 method. 



(b) Stain with aniline- water gentian-violet solu- 

 tion, two to five minutes, warming slightly. 



(c) Add Gram's iodine solution one and one-half 

 minutes. 



