218 TECHNIC, 



(d) Apply alcohol, repeatedly, long as stain contin- 

 ues to come away from the preparation. 



(e) Wash in water and examine as a water mount. 



(f) If desired, dry and mount in balsam. 



GABBETT'S METHOD FOR TUBERCULOSIS, ETC. 



(a) Make a cover-glass smear of the sputum, pus, 

 blood, or urine to be examined. After the preparation 

 is dry, affix by passing three times through the flame. 



(b) Using a Cornet forceps, apply carbol-fuchsin 

 five to ten minutes, heating until steam appears. 



(c) Wash in water. 



(d) Apply acid methylene blue for one minute. 



(e) Wash in water. 



(f) Dry and mount in balsam. 



Staining Tissues for Bacteria. 



Tissues may be stained in Gram's method by the fol- 

 lowing process : 



METHOD FOR STAINING BACTERIA IN SECTIONS. 

 IN SECTIONS. 



(a) Using an aqueous solution of fuchsin, gentian- 

 violet or methylene blue; apply stain for about five 

 minutes. 



(b) Wash in water. 



