Laboratory Outline for General Bacteriology 



From air plates select seven bacterial colonies of different ap- 

 pearances and write descriptions of them in note book. Then make 

 transfers from them to agar slants. Mark tubes so they can be 

 identified with descriptions. To transfer colony sterilize platinum 

 loop by heating entire length of wire to redness in the gas flame. 

 Pass .the lower part of the handle three or four times through the 

 flame. When cool touch loop to the colony to be transferred. 

 Remove cotton plug and hold it so the lower part will not become 

 contaminated by touching anything. Touch the loop to the surface 

 of the agar at the lower part of the tube and make a straight 

 streak up to the top. Care is necessary not to cut down into the 

 agar. Replace cotton stopper. Place tubes in locker to incubate. 



Glossary on pages 57, 58, 59 is to be learned. These terms will 

 be constantly used so it is necessary to become familiar with them 

 now. There will be a written on it soon, probably at the next 

 laboratory period. 



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