Laboratory Outline for General Bacteriology 



35. Do allow agar to harden in plates without tipping them around 

 and breaking the medium. 



Gelatin Stab 



36. Do have needle straight before puncturing. 



37. Do puncture clear to bottom of tube. 



38. Do remove needle in line of puncture. 



39. Do puncture in center of tube and only once. 



40. Do hold tube above medium so warmth of the hand will not 

 melt the gelatin. 



41. Do watch cultures carefully to see when liquefaction begins 

 and when it is complete as well as for the form of growth 

 in both liquefying and non-liquefying cultures. 



Morphology 



42. Do come at right times to make agar slants to have them at 

 the required age for studying at laboratory periods. 



43. Do prepare films with only enough bacteria to show slight 

 cloudiness. 



44. Do spread films over the entire cover glass. 



45. Do leave stains on the exact time called for in the directions. 



46. Do measure at least ten bacteria to find the size of the 

 majority. 



47. Do measure both length and breadth of rod shaped bacteria 

 and put both measurements down. 



48. Do use care to fill out all points called for under "Morphology." 



49. Do examine preparations for motility as soon as possible after 

 making, as many bacteria lose their motility very quickly ; and 

 do shut down on the light enough so bacteria can be seen. 



50. Do handle cover glasses with forceps. 



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