Laboratory Outline for General Bacteriology 



THE CHART 



The purpose of the descriptive chart is to provide a uniform 

 and concise method of recording nearly all the ordinary and some 

 of the unusual observations concerning the morphology and activi- 

 ties of bacteria. It should familiarize students with bacterial char- 

 acteristics and facilitate in the grouping and identification of species. 



For preliminary practice in handling bacteria five pure cultures 

 will be given each student. This work includes the Gram's stain, 

 fuchsin stain for measurement, inoculations into and observations 

 on agar slants, gelatin stabs, nutrient broth, milk, litmus milk, 

 potato, and the nitrite test. 



After this six unknown cultures are given each student. All 

 data called for on the Descriptive Chart is worked out and filled in. 

 When complete the cultures are named by comparing descriptions 

 with those on pages 43, 44, 45, 46. For work on unknown cultures 

 12-14 laboratory periods are given. 



No bacteria pathogenic to man are given out to students in 

 this course. 



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