Laboratory Outline for General Bacteriology 



Odor, absent, decided, resembling 



Consistency, butyrous, viscid, membranous, brittle. 

 Medium, grayed, browned, reddened, blued, greened. 



Gelatin Stab, Incubation temp C, Age days 



Growth, uniform, best at top, best at bottom. 



Line of puncture, filiform, beaded, papillate, villpus, arborescent. 

 Liquefaction, none, crateriform, napiform, infundibuliform, saccate, strati- 

 form, begins in d complete in d. 



Depth of liquefaction in tube of 10 mm. diameter evenly inoculated at 



20C for 30 days mm. 



Medium, fluorescent, browned. 



Potato, Incubation temp C, Age days 



Terms as in Agar stroke. 



Nutrient Broth, Temp C, age days 



Surface growth, ring, pellicle, flocculent membranous, none. 

 Clouding, slight, moderate, strong, transient, persistent, none, fluid turbid. 

 Sediment, compact, flocculent, granular, flaky, viscid on agitation, abun- 

 dant, scant, none. 



Agar Colonies, temp C, Age days 



Growth, slow, rapid. 



Form, punctiform, circular, irregular, mycelioid, filamentous, rhizoid. 

 Surface, smooth, rough, concentrically ringed, radiate. 

 Elevation, flat, effuse, raised, convex, pulvinate, umbonate. 

 Edge, entire, undulate,lob ate, erose, filamentous, curled. 

 Internal structure, amorphous, finely-, coarsely-, granular, filamentous, 

 curled, concentric. 



Gelatin Colonies, temp C, Age days 



Growth, slow, rapid. 



Form, punctiform, circular, irregular, mycelioid, filamentous. 

 Elevation, flat, raised, convex, pulvinate, crateriform, (liquefying) 

 Edge, entire, undulate, lobate, erose, filamentous, floccose, curled. 

 Liquefaction, cup, saucer, spreading. 



Internal structure, amorphous, finely-, coarsely-, granular, filamentous, 

 curled, concentric. 



PHYSIOLOGY 



TEMPERATURE RELATIONS 



Optimum temperature for growth C. 



Maximum temperature for growth C 



Minimum temperature for growth C. 



CHROMOGENESIS 



Nutrient broth 



Nutrient gelatin 



Nutrient agar 



Potato... 



PRODUCTION OF INDOL 



Medium : 



Indol absent, present in days 



PRODUCTION OF HYDROGEN SULFIDE 



Medium : 



H2S absent, present in days 



30 



