Laboratory Outline for General Bacteriology 



AGE OF CULTURES, TO DETERMINE 



Motility 



Flagella 



Spores and sporangia 



Stains for measurement of vegetative cells 



Gram's stain 



24 hours 



24 " 



3 days to 1 week. 



24 hours 



24 " 



AGE OF VARIOUS CULTURES WHEN EXAMINED 



Agar slants 

 Potato slants 

 Nutrient broth 

 Gelatin stabs 



Agar plates 

 Gelatin plates 

 Dunham's peptone 

 for Indol test 

 Lead acetate agar 

 for H S test. 

 Milk " 

 Litmus milk 

 Nitrate broth 

 Nitrate agar 

 Starch agar for 

 diastatic action 



Fermentation 

 tubes of dextrose 

 lactose, sacch- 

 arose, glycerine 



described after 7 days, checked up at 14 days 



same as agar slants 



same as agar slants 



examine every laboratory period until 30 days 



old, recording any changes 

 examine after 1 week 

 from 2 to 7 days 



4 days 



7 days 



1, 2, 4, 7, and 10 days 

 1, 2, 4, 7, and 10 days 

 1, 2, 4, 7, and 10 days 

 1, 2, 4, 7, arid 10 days 



7 days, unless the growth is very rapid, then 

 the test is made sooner. 



examined 2, 7 and 14 days. 



TEMPERATURE OF INCUBATION 



All cultures are to be grown at locker temperature, which is 

 about 20 C. except the one set of agar slants made for testing 

 growth at 37 C. 



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