Laboratory Outline for General Bacteriology 



1 platinum loop 



1 test tube rack 



2 wire baskets 

 1 slide box 



1 ruler 



1 thermometer 



2 pipette cases 

 50 Petri dishes 

 25 Ice pipettes 



6 Ice pipettes graduated to 1/10 cc. 



2 2cc pipettes 



The above list together with the prices is on the inside of the 

 locker door. Check up carefully and if anything is missing report 

 at once. Absolutely no allowance is made for any shortage unless 

 reported at this time. Each student is held responsible for these 

 materials and will be charged at the end of the semester for any- 

 thing broken or missing. Do not leave materials out on the tables 

 or lying around the laboratory. They are returned when the owner- 

 ship is known but in most cases it is not known and the student 

 loses them. 



The Petri dishes or plates in the lockers are sterile so do not 

 open them. When counting pipettes count from the end without 

 taking them from the case, as they are sterilized ready for use. 



Bring a towel or cloth for drying glassware. 



Students are required to wash their Petri dishes and pipettes 

 after using them. Other glassware is cared for by the depart- 

 ment. Care must be used to wash the glassware perfectly clean. 

 If any particles of media remain, sterilizing burns them on and 

 leaves dark brown patches which can only be removed by scraping 

 or by a strong acid cleaning mixture. Petri dishes are to be wiped 

 dry. Mark each one with your locker number. All glassware is 

 marked with red glass marking pencils. These pencils are sold 

 in the laboratory for lOc each. After pipettes are cleaned they 

 are returned to their case with points down. Cases are also marked 

 with the locker number. Place cases of pipettes and Petri dishes 

 which you wish sterilized on the long table in north end of labora- 

 tory. In the book there write your name, locker number, number 

 of dishes, number of cases of pipettes left to be sterilized, and the 

 date. They will be sterilized and returned to your locker. Plates 

 and pipettes must be washed promptly when you are through using 

 them. 



Forms for recording notes will be given out on the board. Use 

 the forms given. Leave one page in the note book for each experi- 

 ment. 



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