Laboratory Outline for General Bacteriology 



Lactose fermentation tubes 31 



Lead acetate agar 34, 39 



Light, effect on bacteria 25, 38 



for microscopic work 11 



Lips, bacteria on 54 



Liquefaction of gelatin 32, 33 



types of 33 



Litmus milk 40 



reduction of 40 



Lockers, equipment of 14, 15 



Loeffler's methylene blue 35 



M 



Magnification 12 



Meats, bacteria in 55 



Media, liquid 24, 25 



nutrient 38 



solid 37 



Method for determining the per 

 cent of bacteria killed by sun- 

 light 38 



Methylene blue 35 



Micrometer scale 11 



Measurement of bacteria 12 



Micrococcus agilis 12, 43 



Micrococcus lactis varians 43 



Microscope 11 



Abbe condenser 11 



cleaning of 11 



how to use 11 



lighting for 11 



magnification 12 



mirror of 11 



Milk, dilutions for plating ... .50, 51 

 direct microscopic examination . . 52 



effect of temperature on 53 



peptonization of 32, 40 



reaction of 40 



staining of 52 



Mirror, concave 11 



plane 11 



Molds 19 



Mordant for flagella staining 3~ 



Morphology 36 



Motility 16, 17 



Mouths of culture tubes, flasks, 



sterilization of 22, 23 



N 



Nitrate agar 38 



Nitrate broth 38 



test solution for 39 



Nitrites, test solution for .39 



Nutrient broth . .38 



O 



Objectives, oil immersion 11 



cleaning of 12 



Oil, cedar 11 



immersion . .11 



Paradimethylaminobenzaldehyde . .39 



Pencils, bacteria on 53 



Peptone 39 



Peptonization of milk 40 



Pipette 15, 49 



Plates, agar 38 



gelatin 38 



Plate cultures, examination of . .50, 51 



Plating 24,25, 26, 27 



Platinum needle, sterilization of... 14 

 Potato 



preparation of 37 



types of growth on 33 



Pressure, of steam in sterilization . .14 

 Prototrophic 59 



R 



Reaction 59 



Reduction of litmus 40 



Rennet curd 31, 59 



Resistance of spores 55 



Results, interpretation of 51 



Rhodoccus roseus 43 



Rules for use of microscope 11 



S 



Saccharose fermentation tubes ... .31 



Sarcina 



aurantiaca 43 



flava 43 



lutea 43 



Slanecii 43 



Sneeze, bacteria disseminated by . . 54 



Spores, testing for presence of ... .55 



Staining 



flagella 37 



Gram's method 35 



Stains, preparation of 3^ 



Staphlococcus albus 43 



Starch, agar 



test for diastatic action of bac- 

 teria on 40 



Steam, sterilization by 14 



Sterilization 



by dry heat 14 



by gas flame 14 



by steam under pressure 14 



62 



