CLARK: CYTOLOGY OF CARCINOMA. 225 



of resting cells, B, B', figure 8, etc., just outside the zone 

 of "chromatic cells" H, H', H". Its chromatin had wholly 

 or largely disappeared, and if any remained it was loosely 

 diffused through the karyoplasm. Cell E is another cell show- 

 ing no chromatin in any of the segments of its nucleus. It 

 evidently was in decay for it was very translucent. The frag- 

 ments of its nucleus showed no chromatin at all. It may be that 

 a cell such as B, which has vigor enough to cause its nucleus 

 to segment but not to cytoplasm, gradually loses its staining 

 ability and its definiteness of outline and later becomes merely 

 a mass of debris through a stage represented in cell E. Cells 

 F and G are two cells in the indirect division process. The di- 

 rectly dividing cells are much larger than the resting cells or 

 those dividing indirectly. Cell A is .0093 mm. in its greatest 

 diameter, cell B is .007 mm., cell C is .0093 mm., cell G is .0062 

 mm., cell F is .0062 mm., cell E is .0093 mm., cell D is .0085 

 mm., while cell I, an average resting cell, is .0062 mm. in its 

 greatest diameter. This cell shows the breaking up of the 

 chromatin and its diffusion through the karyoplasm, possibly 

 preceding cells H, H', H". Its nucleus is .005 mm. in diameter, 

 while the diameter of the "chromatic mass" in cells H, H', H" 

 is .0031 mm., showing evidently a shrinkage in the latter. 



Figure 14. In this section two indirect divisions are- shown 

 located on the very edge of the necrotic plug, and two more 

 near. There are also close to the edge of the nest three direct 

 divisions, still showing a greater proportion of direct divisions 

 near the necrotic area than indirect, when compared with the 

 total number of divisions of each in the section. But this 

 shows that indirect division may occur on the very edge of a 

 degenerating area. 



At the lower extremity of the limb of the regular section 

 following is found a circular group of cells, which in the next 

 section shows a connection with the larger body. This is the 

 first appearance of this group, but in the previous sections 

 there is a circular space indicating where it has been but from 

 which it has in some way been lost. There is a cleft entirely 

 through the regular section, but this was where the cells had 

 pulled apart and not where they had degenerated. The direct 

 divisions are intimately associated with the necrotic spots. In 

 the middle segment there are two indirect divisions on the edge 



