734 CHARLES PAUL ALEXANDER 



The more important recorded protozoan parasites are as follows: 



Class, Sporozoa. 

 Subclass, Telosporidia. 

 Order, Coccidiomorpha. 

 Family, Adeleidae. 



1. Adelea tipidae Leger. In the intestine of species of Tipula. 

 Order, Gregarinida. 



Family, Gregarinidae. 



2. Hirmocystis polymorpha (Le"ger, 1892: 113). In the intestine of the larva of Limnobia sp. 



3. H. ventricosa (Leger, 1892 :111). In the alimentary canal of Tipula oleracea, Nephrotoma 

 pratensis, and other species. 



4. Gregarina longa (Leger, 1892:117). In the alimentary canal of a species of Tipula. 

 Family, Actinocephalidae. 



5. Actinocephalus tipulae Leger (1892:141). In the alimentary canal of Tipula larvae. 

 Probably the same species has been recorded from the larvae of a species of Ctenophora. 



6. Pileocephalus striatus Leger & Duboscq (1909:887-893). In the mid-intestinal epithelium 

 of the larva of Ptychoptera contaminate,. The Pileocephalus live in the epithelium of the 

 mid-intestine, attaching themselves to the epithelial cells and hypertrophying the adjoin- 

 ing tissues. They obtain their nutriment from the food that penetrates into the cells. 



Family, Stylorhynchidae. 



7. Near Stylorhynchus (Miall, 1893:237). In the stomach of larvae of Dicranota bimaculata. 



Subclass, Neosporidia. 

 Order, Cnidosporidia. 

 Suborder, Microsporidia. 

 Family, Nosematidae. 



8. Nosema strictum Monz. (Moniez, 1887). In muscles, conjunctive tissue, and other parts 

 of Nephrotoma pratensis. 



9. Gurleya francottei Le"ger & Duboscq (1909:894). In the epithelium of the mid-intestine 

 of the larva of Ptychoptera contaminata. 



Class, Flagellata. 



Family, Trypanosomidae (Herpetomonadidae). 



10. Crithidia campanulata Leger. At the juncture of the mid- and hind-intestines in the 

 larva of Ptychoptera contaminata (Leger & Duboscq, 1909:898-900). 



The writer is indebted to Dr. R. Kudo for assistance in determining the 

 terminology used above. 



Bacteria are frequently found in crane-fly larvae. Leger and Duboscq 

 (1909:900-901) record undetermined spirochaetes in the epithelial cells 

 of the posterior part of the mid-intestine of Ptychoptera contaminata. 

 Dr. Hugh Glasgow, of the Geneva Experiment Station, informs the writer 

 that in Illinois a large tipulid larva, probably that of Tipula abdominalis, 

 living in the leaf-drift of prairie streams, is heavily infested with bacteria. 

 Most of the specimens observed had an abundance of small coccus and 

 spirochaete forms, with occasional specimens of a gigantic bacillus measur- 

 ing from forty to eighty microns in length and disporous. These large 

 bacilli infest the hind-gut of the larva. 



