492 APPENDIX. 



which weaker ones can readily be prepared when re- 

 quired. 



Preparations should be placed in - i per cent, solution 

 for the first 24 hours, that being ultimately replaced for a 

 similar period by one of '5 per cent, which should be 

 changed if necessary. When well hardened they should 

 be transferred to 75 per cent, alcohol. 



When used for purposes of decalcifkation the maxi- 

 mum quantity should be employed, and to it a few drops 

 of nitric acid may be added. 



8. Corrosive sublimate, Solution of. 



To a saturated aqueous solution add a few drops of 

 acetic acid. 



A relatively large quantity of the above must be em- 

 , ployed, and the preparation, after at most an hour's 

 immersion therein, should be well washed under running 

 water before being transferred to alcohol. 



9. Eosin, Solution of. 



Aqueous and alcoholic solutions should be prepared ; 

 the former for use with fresh material, the latter with 

 that which has been previously hardened. 



10. Glycerine. 



By 'weak glycerine,' referred to in the text, is meant a 

 solution composed of equal parts in bulk of glycerine 

 and distilled water. 



1 1. Gold chloride, Solution of* 



A i per cent, solution is customarily employed. 



Tissues submitted to the action of this reagent should 

 be kept free of contact with metal. 



1 2. Hsematoxylin, Solution of. 



a. Prepare a saturated solution of crystallized calcic 

 chloride in 70 per cent, alcohol ; then add alum to 

 saturation. 



