260 METHODS OF ANALYSIS. 



minutes with 25 cc of petroleum ether and transfer the liquid to a beaker, rinsing the 

 residue with a little fresh petroleum ether. Add to the beaker 20 cc of water and 

 evaporate the petroleum ether. Gently warm the first residue with 10 cc of water, 

 cool, and filter the solution through a small filter into a separator, rinsing the vessel 

 and filter with several small portions of water, and finally with the water in the beaker. 

 Shake out the liquid with four portions of 10 cc each of chloroform and evaporate the 

 latter in a tared dish. Add 3 cc of alcohol and evaporate, repeat the operation and 

 dry the residue to constant weight at a temperature not exceeding 70 C. Report 

 weight of alkaloidal residue. 



COLCHICUM SEED. 



Method I. U. S. Pharmacopoeia VIII, p. 112. Report weight of alkaloidal residue. 

 Method //.See Colchicum Conn. Method II. 



