Nitrogen Metabolism of Bacteria 



33 



After that the amount seemed to suffer some decrease. In the case 

 of B. pyocyaneus and B. subtilis a maximum is not shown in the time 

 of the experiment. The creatinin content in the culture of Staph, 

 pyogenes shows a continuous increase, but its creatin content falls 

 nearly to zero after the second day. 



TABLE 20 

 Formation of Creatinin in Peptone Cultures of Micro-organisms 



Culture 



B. proteus-vulgaris 



B. pyocyaneus 



B. typhosus 



B. coll-communis 



Sp. cholerae 



B. subtilis 



B. paratyphosus 



B. cloacae 



B. faecalis-alkaligenes. 



Sp. metchnikovii 



Staph, pyogenes 



B. dysenteriae, Shiga.. 



B. acidi-lactici 



B. prodigiosus 



Sterile controls 



* The numbers 1 and 2 refer to the glucose-free and the glucose-containing cultures, 

 respectively. 



TABLE 21 

 Formation of Creatin in Peptone Cultures of Micro-organisms 



Culture 



B. proteus-vulgaris 



B. pyocyaneus 



B. typhosus 



B. coli-communis 



Sp. cholerae 



B. subtilis 



B. paratyphosus 



B. cloacae 



B. faecalis-alkaligenes. 



Sp. metchnikovii 



Staph, pyogenes 



B. dysenteriae, Shiga.. 



B. acidi-lactici 



B. prodigiosus 



Sterile controls 



8 Days Old 



5.9 



4.7 

 72 



5.3 

 t 

 5.5 

 5.3 

 6.5 



*«!6" 

 8.8 



0.4 



0.8 



14 Days Old 



* The numbers 1 and 2 refer to the glucose-free and the glucose-containiDg cultures, 

 respectively. 



f When the reagent was added to these samples, a very dark color was produced, which 

 could not be matched with the standard creatinin solution. 



There seem to be two possible explanations of this surprising effect 

 of glucose on creatin- and creatinin-production by micro-organisms. 

 Either these substances are regularly produced by bacteria in peptone 

 solutions and their decomposition as fast as formed prevented by the 

 presence of sugar, or else glucose, o;- some of its split products, actually 

 takes part in the synthesis by the organisms of these two nitrogenous 



