THE CULTIVATION OF BACTERIA. 57 



What are the usual decolorizing agents used ? 



What is Gram's method ? 



Describe Ziehl's carbol-fuchsin method. 



Describe Gabbett's method. 



Give Welch's method of staining the capsule of bacteria. 



Give Johne's method. 



Give Abbott's method of staining spores. Moeller's method. Fiocca's 

 method. 



Give Loeffler's method of staining flagella. 



Which bacteria require the addition of acid to the mordant in order to 

 stain their flagella? Which require the addition of alkalies? 



Describe Bunge's method of staining flagella. Pi tfi eld's method. 



How would you stain bacteria in tissue? Give Gram's method. Weigert's 

 method. Kuhne's method. Ziehl-Neelseri's method. 



Give directions for making Wright's blood-stain. 



Give directions for using same. 



CHAPTER III. 



THE PROCESS, MEDIA, AND UTENSILS OF THE CULTI- 

 VATION OF BACTERIA. 



THE PROCESS OF THE CULTIVATION OF BACTERIA. 



As mentioned before, bacteria can not be separated from 

 one another by form and appearance under the microscope 

 only. Indeed, in a number of instances, and even with the 

 highest power of the microscope, some very inoffensive bac- 

 teria resemble very much and can not be differentiated from 

 some that are highly pathogenic. Especially is this the case 

 with the group of cocci. 



In all such cases it is necessary to study the properties 

 and mode of growth, and for this purpose the bacteriologist 

 must use and prepare suitable soils, which are known by the 

 name of culture-media. These culture-media must themselves 

 be absolutely free from all live bacteria that is, sterile ; or, 

 if they naturally contain bacteria, or if bacteria have been 

 introduced during their preparation, these must be destroyed, 

 or, in bacteriological language, the media must be sterilized. 



THE MEDIA FOR THE CULTIVATION OF BACTERIA. 



All substances that contain carbon and nitrogen compounds in 

 assimilable form associated with water may be used as culture- 



