GONORR1KEA. 115 



or in bouillon. The most commonly used medium for the 

 growth of the gonoeocci is that of Wertheim. It consists of 

 three parts of meat infusion agar mixed with one part of 

 imcoagulated ascitic fluid, hydrocele fluid, or blood serum. 

 The agar is melted and mixed with the fluid or serum at 

 about blood temperature. 



Blood smeared over agar as for the B. influenzas makes a 

 good medium. 



It stains with the basic anilin dyes, especially with gentian- 

 violet. It does not stain by the Gram method. This is a 

 valuable point to differentiate it from the pus cocci, which all 

 stain by the Gram method. 



Pathogenesis. Toure succeeded in causing urethritis in dogs 

 by injecting into their urethras cultures in acid media. Finger 

 and G-ohin have caused acute urethritis, which rapidly disap- 

 peared, by intra-articular injections of cultures into dogs and 

 rabbits. Pus containing the gonoeocci when inoculated into 

 man have reproduced the disease in many instances. Pus 

 cultures of the gonoeocci have also given positive results in 

 many cases : Wertheim, 5 times in 5 cases ; Bockardt, 6 times 

 in 10 cases; Finger, 3 times in 14 cases. Subcutaneous in- 

 jections of the culture produce considerable tumefaction and 

 redness at the point of inoculation, but no abscess-formation. 



VII. Micrococcus Catarrhalis. 



The Micrococcus catarrhaiw is a commonly found organ- 

 ism, occurring in inflammations of the upper respiratory tract. 



Morphology and Staining. It is identical with the Micro- 

 coccus gonorrhoeas in its shape and staining qualities. 



Biology. It can be differentiated by the fact that M. catar- 

 rhalis grows readily on all culture-media. From the inenin- 

 gococcus it can be differentiated by its growth at room tem- 

 perature (below 25 C. and even below 20 G), and by its 

 white colonies. 



Micrococcus catarrhalis does not ferment any sugars. 

 Micrococcus meningitidis ferments dextrose and maltose. 

 Micrococcus gonorrhoeas ferments dextrose alone. 



