TETANUS, MALIGNANT (EDEMA, ETC. 145 



Describe the staining of this bacteria. 



What characterizes cultures of the diphtheria bacillus? 



How are false or pseudobacilli differentiated from true diphtheria bacilli? 



Describe the Neisser method of staining the diphtheria bacilli. 



How does it behave in the presence of oxygen? 



Is it motile ? 



Has it flagella ? 



Does it contain spores? 



At what temperature does it grow? 



What is its thermal death-point ? 



How does it behave in the presence of disinfectants? 



How is it affected by direct sunlight? 



How does it behave in the albuminous fluid? How in the dark? 



How is Loefller's blood-serum for the culture of the Bacillus diphtherise 

 prepared ? 



Describe the growth of this bacillus on Loefller's medium, in bouillon, on 

 gelatin, on agar, on potato, in milk? 



Why is diphtheria a toxic disease? 



How is the toxin of diphtheria obtained? 



Give the effects of inoculation of diphtheria bacilli on guinea-pigs. 



What is the effect of the inoculation of those bacteria on mucous surfaces 

 of animals? 



How do the different cultures of Bacillus diphtherise vary as to their 

 virulence ? 



Give the boards' of health measures for diagnosing diphtheria by means 

 of cultures. 



How is the inoculation of cultures made in those cases? 



What are the sources of error in this form of examination? 



What two forms of pseudobacilli are found ? 



How are they recognized from true virulent bacilli ? 



How is the toxin prepared ? 



How is it gauged ? 



What is constant diphtheria toxin? 



What is the result of the antitoxin treatment of diphtheria? 



What is the result of its prophylactic use ? 



What dose should be given as a prophylactic? 



What dose should be given in the treatment of diphtheria cases? 



CHAPTER XV. 



TETANUS, MALIGNANT (EDEMA, AND SYMPTOMATIC 



ANTHRAX. 



TETANUS. 

 Bacillus Tetani. 



History. Bacillus tetani was discovered by Nicolaier in 1884, 

 and cultivated by Kitasato in 1889. 

 10 M. B. 



