154 



TETANUS, MALIGNANT (EDEMA, ETC. 



as globules which cause liquefaction of the gelatin and 

 coalesce into irregular tabulated liquid areas. The dried 

 spores retain their vitality for months. They resist a tem- 

 perature of 80 C. for one hour, but five minutes' exposure 

 at 100 G. is sufficient to destroy them. Carbolic acid 

 (5 per cent.) is not effective as a disinfectant in less than ten 

 hours. The vegetative form, however, is killed in from three 



FIG. 62. 



Bacillus of symptomatic anthrax: A, vegetative stage gelatin culture; 

 B, spore-forms agar-agar culture. (Abbott.) 



to five minutes. Bichloride of mercury (1 : 1000) will kill 

 the spores in two hours. 



Pathogenesis. Cattle, sheep, goats, guinea-pigs, and mice 

 are susceptible animals. Horses, asses, and rats show only 

 slight local swelling, but no general infection. Dogs, cats, 

 rabbits, chickens, pigeons, and hogs are immune. Inocula- 

 tions are generally made deep into the subcutaneous tissue 

 either with pure cultures of the microorganisms or from bits 

 of tissue of a suspected animal. The symptoms are a rise of 

 temperature, followed by painful swelling at the point of 

 inoculation. Death takes place in from one to two days. 



