QUESTIONS. 155 



The autopsy reveals an extensive swelling of the subcutane- 

 ous tissues with emphysema. The oedematous fluid is blood- 

 stained, and the muscles are dark and prominent. The 

 lymphatic glands are involved. The internal organs show 

 little change. In the fluid of the oedema the bacilli are found 

 in large numbers, lying singly. Early autopsy reveals no 

 bacteria in the blood, only a few in the internal organs. 

 Late autopsy shows a considerable quantity of organisms that 

 have invaded the whole body. The bacilli in the body arc- 

 found to contain spores. This serves as a differentiation, 

 in addition to other points, between it and the Bacillus 

 anthraeis. 



Immunity. One attack of the disease if not fatal affords 

 protection against future attacks. The opposite of this hap- 

 pens with malignant oedema, one attack of which seems to 

 predispose to other attacks. 



QUESTIONS. 



When, where, and by whom was the Bacillus tetani discovered and culti- 

 vated? 



How many forms of the Bacillus tetani are there, and how are these dis- 

 tinguished ? 



What is the characteristic appearance of the spore-bearing form ? 



In what atmosphere does it grow best, and why? 



What is the temperature-limit of its growth ? 



How does it grow in gelatin ? In agar? In bouillon? In milk? 



What is Kitasato's method of obtaining pure cultures of this bacillus? 



In what form is it motile ? 



What agents and chemicals are the spores capable of resisting, and to what 

 extent? 



What is tetanin? 



Describe a method of growing anaerobic bacilli with the use of paraffin oil. 



What animals are susceptible to the infection of tetanus? 



Describe the autopsy of an animal inoculated with Bacillus tetani. 



Where and how are inoculations in animals made? 



What symptoms are produced by tetanin injection? 



What type of infection is tetanus ? 



How is tetanus toxin prepared? 



What is the degree of toxieity of tetanin? 



How is the antitoxin of tetanus prepared? 



How is it used and for what purpose? 



Why is it of more use in veterinary than in human medicine? 



How is the strength of tetanus antitoxin expressed? 



By whom was discovered the bacillus of malignant oedema? 



Where is it found ? 



What is its appearance ? 



