172 Royal Society : — 



Subsequent appearances of the infusion. — The infusion in all the 

 tubes was found on examination from day to day to retain its lim- 

 pidity. Subsequent microscopic examination of all the tubes at 

 various periods subsequent to the closure of the tubes (from four 

 days to three weeks) yielded no indication whatever of a deve- 

 lopment of Bacteria or other organisms, nor of any change. A 

 portion of the same infusion placed in an uncleansed beaker for 

 comparison was milky and swarming with Bacteria after three 

 days. 



Series C Nov. 28th. Experiments unth turnip~and-cheese infu- 

 sion. — The infusion similar in all respects to that in series B, but 

 prepared -ndth a somewhat larger proportion of turnips ; there- 

 fore of higher specific gra\dty, which was not numerically deter- 

 mined. 



Tubes 20, 21, 22, 23. Boiled and sealed approximately during 

 ebuUition. Not subsequently submerged. 



Tubes 24, 25. Boiled and sealed approximately during ebullition. 

 Subsequently submerged in boUing water during thirty minutes. 

 The tubes were preserved in the air-bath as in Series A and B. 

 Series D. Nov. 30th. — An infusion prepared as in Series B and 

 C, but brought to a sp. gr. 1031 by evaporation after filtration. 



Tubes 26, 27, 28, 29. Sealed cold. Subsequently submerged in 

 boiling water for thirty minutes. 



Tubes 30, 31. Boiled and sealed approximately during ebullition. 

 Not subsequently immersed. 



Tubes 32, 33. Boiled and sealed approximately during ebulhtion. 

 Subseqviently submerged in boiling water for thirty minutes. 



Appearances in the infusions, Series C and D, at the time of sealing 

 and submerging. — The appearances in the freshly prepared infusion 

 were similar to those described above as characterizing such infu- 

 sions. 



Subsequent naked-eye examination of the tubes did not reveal 

 the slightest change ; they remained limpid. Specimens from each 

 group were opened and examined ^^ith the microscope after four 

 days, and the microscopic characters found to be unchanged : the 

 liquid was perfectly sweet. The remaining tubes were examined 

 at intervals before the end of December, being maintained during 

 the whole time at a temperature of 35° to 40° C. in the air-bath ; 

 they equally proved to have remained unchanged when, opened and 

 examined with the microscope, and were also free from unpleasant 

 smell. 



Series E. Nov. 28th. — Six porcelain capsules were heated to 

 redness, and nearly filled Trtith the turnip-infusion used in Series C. 

 They were placed on the air-bath under a glass shade. 

 Capsules 1, 2. The infusion was unboUed. 

 Capsule 3. The infusion was boiled in the capsule. 

 Capsule 4. The infusion was introduced after it had been boiled 

 for five minutes in a superheated test-tube. 



Capsules 5 and 6. The infusion was that used in capsule 4, but 

 a drop of distilled water was added to each of these two capsules. 



