TABLE OF CONTENTS. Vll 



PAGE 



Observations on budding-fungi by Bail, Reess, Zopf, and 



Brefeld .. : 120 



Chemical investigations by Rayman and Kruis . . . . 120 121 



HANSEN'S INVESTIGATIONS . . . . . . . . . . 122 159 



General remarks . . . . . . . . . . . . 122 



1. Preparation of the Pure Culture 122123 



2. The Analysis 124158 



(a) The Microscopic Appearance of the Sedimentary 



Yeast 124125 



(6) Formation of Ascospores . . . . . . . . 125 137 



(c) The Formation of Films 137142 



(d) The Temperature Limits for the Saccharomycetes 142 143 



(e) Cultivation on a Solid Nutritive Medium . . 143 144 

 (/) The Behaviour of the Saccharomycetes and 



Similar Fungi towards the Carbohydrates and 

 other Constituents of the Nutritive Liquid 



Diseases in Beer . . . . . . . . 145 151 



(y} Variations in the species of Saccharomycetes . . 151 156 

 (A) Gelatinous Formation secreted by the" Budding- 

 fungi 156158 



The Microscopic Appearance of a Yeast-cell . . . . . . 158 159 



Classification of the Genus Saccharomyces . . . . . . 159 188 



Saccharomyces Cerevisiae I. Hansen . . . . . . . . 159 161 



Saccharomyces Pastorianus I. Hansen . . . . . . 161 165 



Saccharomyces Pastorianus II. Hansen . . . . . . 165 167 



Saccharomyces Pastorianus III. Hansen . . . . . . 167 170 



Saccharomyces ellipsoideus I. Hansen . . . . . . . . 170 172 



Sacchuromyces ellipsoideus II. Hansen . . . , . . 173 174 



Two ellipsoid yeasts described by Will . . . . . . . . 174 175 



Saccharomyces Ilicis. Gronlund . . . . . . . . 175 



Saccharomyces Aquifolii. Gronlund . . . . . . . . 175 



Saccharomyces pyriforrnis. Marshall Ward . . . . . . 175 



Saccharomyces Marxianus. Hansen . . . . . . . . 176 



Saccharomyces exiguus (Reess). Hansen . . . . . . 176 177 



Saccharomyces membransefaciens. Hansen . . . . . . 177 178 



Saccharomyces Hansenii. Zopf . . . . ... . . . . 178 



Saccharomyces Ludwigii. Hansen . . . . . . . . 179 180 



Saccharomyces acidi lactici. Grotenfelt . . . . . . 180 



Saccharomyces minor. Engel . . . . . . . . . . 180 



Saccharomyces anomalus. Hansen . , . . . . . . 181 182 



Saccharomyces conglomerates. Reess . . . . . . . . 182 183 



Carlsberg bottom-yeast No. 1 and No. 2 . . . . . . 184 186 



Grouping of cultivated yeasts for practical purposes . . . . 186 187 



Observations made by Irmisch . . . . . . . . . . 187 



