128 



MICRO-ORGANISMS AND FERMENTATION. 



Budding can occur at any point on the surface of the 

 swollen spores ; this budding usually takes place after the 

 wall of the mother-cell has become ruptured or dissolved, but 

 it also occasionally takes place within the mother-cell. After 

 the buds have formed, the spores can still remain connected, 

 or they can soon become detached from each other. 



An especially curious and exceptional case is when the 

 wall separating two spores becomes dissolved, so that a fusion 



FIG. 28. 



Budding of the spores in Saccharomyces cerevisise I., after Han sen : a, three 

 spores without the wall of mother-cell ; &, cell with four spores ; at b' 

 the wall of mother-cell is ruptured ; c, cell with four spores, three of 

 which are visible ; at c' and c" the ruptured wall of mother-cell is seen ; 

 d, cell with three spores, at d'" the ruptured wall of mother-cell ; 

 e e'"" development of a very strong colony ; / Ji, other forms of 

 development ; at h" the wall between the two spores has disappeared. 



of the spores results (see Fig. 28, e e'"" and h h"). 

 Hansen assumes that the biological significance of this 

 phenomenon is that the spores, placed under unfavourable 

 conditions, have a greater chance of forming buds. One 

 spore plays the part of a parasite to the other. The growing 



