168 MICRO-ORGANISMS AND FERMENTATION. 



one of the species which produce this disease. Recent experi- 

 ments of Ha/nsen show that this disease-yeast possesses another 

 peculiar property ; namely, when the fermenting wort has an 

 opalescent appearance, the addition of Sacch. Pcistorianus III. 

 will in certain cases effect a clarification. 



Ascospore formation (Fig. 32, 4) : 

 At 29 C. no ascospores are developed. 

 27 28 the first indications are seen after 35 hours. 

 ,, 2b*5 ,, ,, 60 



9;? 90 



55 ^^ 55 55 5? 55 55 ^ 55 



)5 ^2 ,, ,, -9 



n44 

 55 55 5> 55 5? 55 



55 16 ,, ., 53 ,, 



10*5,, 7 days. 



?5 "'^ 55 55 55 5? 55 " y> 



4 no ascospores are developed. 



Size of the spores 2 5 p. 



Film-formation : 



At 34 C. no film-formation occurs. 

 26 28 feebly -developed film-specks are 



seen after 7 10 days. 



9H 99 Q 1 *> 



55 ^ U -^ 55 55 55 55 55 " ^ " 55 



13-15 } r 1020 



67 (Fig. 42) 1 2 months. 



5? 3 o / ,, I. o o ,. 



2 3 no film-formation occurs. 



Microscopic appearance of the cells in the films : 



At 20 28 C. : Nearly the same forms as in the sediment- 

 ary yeast. 



At 15 3 C. : Strongly-developed colonies of elongated 

 sausage-shaped or thread-like cells, which closely resemble a 

 mycelium in appearance (Fig. 42). 



In old cultures of films, the cells have the same forms as 

 at 15 3 C. 5 and are often still thinner and more thread- 

 like. 



