172 MICRO-ORGANISMS AND FERMENTATION. 



Film-formation : 



At 38 C. no film-formation occurs. 



33 34 feebly-developed film-specks are 



seen after 8 12 days. 



26 28 9 16 



20-22 1017 



1315 (Fig. 44) 1530 



6 7 2 3 months. 



5 no film-formation occurs. 



Microscopic appearance of the cells in the films : 

 At 2034 C. and 67 C., the cells are smaller and 

 more sausage-shaped than in the sedimentary yeast. 



FIG. 45. 



Saccliaromyces ellipsoideus II. Hansen. Cell-forms of young sedimentary 

 yeast, after Hansen. 



At 13 15 C., freely-branched and strongly-developed 

 colonies of short or long sausage-shaped cells, often with 

 verticillated branches (Fig. 44). 



In old cultures of films, the cell forms are the same as 

 at 1315 C. 



Streak cultures of this species in wort-gelatine (wort with 

 the addition of about 5*5 per cent, of gelatine) give in 

 contradistinction to the other five species in the course of 

 eleven to fourteen days at 25 C., a characteristic net-like 

 structure, by means of which it can be distinguished by the 

 naked eye from other species. 



