CH. VII] 



PHOTOGRAPHING WITH THE MICROSCOPE 



229 



.Exc 



FIG. 135. VIEW OF THE BACK LENS 

 OF THE OBJECTIVE, SHOWING THE CON- 

 DENSER OUT OF CENTER AND CENTERED. 



Exc The spot of light (Z>) is to one 

 side of the center, showing that the optic 

 axis of the condenser is not in line with 

 that of the microscope. 



C The spot of light (D) is in the center, 

 showing that the optic axis of the con- 

 denser and microscope are in line. 



A plan used by many skilled workers is to light the object and the 

 field around it well and then to place a metal diaphragm of the proper 

 size in the camera very close 

 to the plate holder. This will 

 insure a clean, sharp margin 

 to the picture. This metal dia- 

 phragm must be removed while 

 focusing the diaphragm of the 

 projection ocular, as the dia- 

 phragm opening is smaller than 

 the image of the ocular dia- 

 phragm. 



If the young photo-micfog- 

 rapher will be careful to select 

 for his first trials objects of 

 which really good photo-mi- 

 crographs have already been 

 made, and then persists with each one until fairly good results are 

 attained, his progress will be far more rapid than as if poor pictures 



of many different things were 

 made. He should, of course, 

 begin with low magnifications. 

 353. Adjusting the objec- 

 tive for cover-glass. After 

 the object is properly lighted, 

 the objective, if adjustable, 

 must be corrected for the 

 thickness of cover. If one 

 knows the exact thickness of 

 the cover and the objective is 

 marked for different thick- 

 nesses, it is easy to get the 

 adjustment approximately cor- 

 rect mechanically; then the 

 final corrections depend on the skill and judgment of the worker. 

 It is to be noted too that if the objective is to be used without a 



Exc 



FIG. 136. FIELD OF THE MICROSCOPE 

 SHOWING THE LlGHT IN THE CENTER AND 

 TO ONE SIDE. 



C Fl The light is in the center and 

 illuminates the object. 



Exc Fl The light is at one side of the 

 center and does not illuminate the object. 

 (The field is not fully lighted, as a low 

 power is used to center the object and 

 the light). 



