ANALYSIS (>! MoTNTINfi M KTIH >!>S 457 



:i|i:irt IVnin it hy :i spring. so MS In close |,\ the | .ressn n - (,fl|n. 

 linger ;ilid to open of itself, \\ill In- found (if j| H . M: M |e.- ) \\ell 

 sharpened) much superior to :\\\y kind of kni\es for cutting throu:_:h 



delicate tissues with MS little disturlunce of tii<>m MS possihle. A 



I'.-iir ol' sni.-ill slr.-ii-lit forceps \\itli line points. Mini .-mother pnirof 

 curved forceps. will !H> found useful in addition to tin- oi-ilin.-n v 

 dissect inu forceps. 



( )f :ill 1 lie i list rn nielits ci u it ri \ e<| for < lei ic:it e d issect ioi is, lio\\ i '\ er. 

 fe\\ :il'e more sel'\ ice;i I le tlliili tliox- \\liicli tin- In icl'oscopist III.MV 

 in.-ike for himself out of ordin.-irv tti-<-(//cx. These should he lixed in 

 liirllt \\ ..... lell h;illdles (the 

 cednr sticks used for 

 c.-iliiel h:iir pencils, or t he 

 h.-indles of steel pen 

 holders, or siniill porcii l-'n.. :IHH. Spi-ii. 



pine (piills will answer 



extremely \\ell) in such ;i in:iimer that their points slionld not 

 project i:ir. since they will other\\i>e h;i\e too lunch 'spring;' 

 much IIKIV l)edoiieli\ t hei r mere fi-nriiiif ;ict ion : hut if it be desired 

 to use them '.\srnffinif inst rnineiit s. .-ill tlint is neces>;i ry is to Imrden 

 :ilid temper them. :illd t hen ; 'i \ e them ;in ed^e Ujioil .M liolie. It U i 1 1 

 solliet ilnes he desir.'lhle to ^i\e ;i liner |ioint to such needles thiili 

 they oi-i^iiuilly possess; this ;dso ni:iy he done Upon ;i holie. A 

 needle \\ith its point In-lit, ton ri^ht .-in^le. or m-.-irly so. is often use 

 fill ; :nnl this iimy he shnped hy simply he;itini: the point in M l:imp 

 or c:indle. ^hini; to it the recpiired turn with :i p:iir <>f pliers. :ml 

 then h:n deiiin-- the point ;i^:iin hy re he.-itin^ it :ind |ilinii:iMir it into 



cold \\;i!er or t;illo\\ . 



Analysis of Methods of Preparation and Mounting which 

 follow . 



1. Descriptions of microtomo. ;md knife-holdere :iul /////'' 



position. 



2. Mounting objects in i;-eiier:d. 



.'5. I 'rep.-i ration of wj't tissues, under the following Subtitles : 



Fixation, 



I )ehdr:it ion. 



Staining, 

 This lust is further subdivided .-is follo\\s: 



Sl;iili- for li\ ilii:' ohjects. 



St;iins for fresh tissues. 



St.-iins for lixed :ind preserved entire ohjects. 



Nucle.-ir st. -i ins for >ect ions. 



lM:ism:it ic st.-iin-. 



[mbed,ding methods under the following subtitles : 



Imheddin^ methods in ^eiier;d. 

 'Die p;ir;illin method. 



This lust is further subdivided MS follows: 

 1. S.-iturMtion with :i solvent. 

 -. SM! iir.-it ion with p:ir.Mtlin. 



