(HLMICAL BASIS <>F Till. ANIMAL UnhV. I'.t 



The chief though somewhat unsatisfactory evidence which is 

 advanced as to the difference of the two products is the following: 



1. Al]tali-albuniin_is_in general more soluble than acid-albumin. 



2. Wh.-ii pi-eclated h^ neutralisation tin- former (ajjj^li) i* 

 t. the latter (acid) iX n ]I e viscid, transparent. an 



atino us. 



3. \Vhen dissolved in a lainiinum of alkali and heated to 

 00 in scaled tubes, alkali-albumin coagulates. .-u-jd-allmimp 



4. When alkali-albumin is dissolved in Na a HP0 4 it js iu,f pp.- 

 cjpitated on the addition of an acid until aTTTTie salt has IK-CM 

 converted into XaH-iHV (Of. above, p. 17.) 



Acid-albumin can IK- coiivertc^l into alkali-albirmjn }j\- tht; 

 actjiui of strong alkalis, but the rcycr.-c conversion of tin- imuhjpt 

 thus obtained or of an ordinarily prepared alkali-nlhiiinin into 

 acid-albumin is stated to b 



'I'hc rotatory ]io\vcr of alkali-albumin varies accnrdinu' t<- n- 

 source; thus when prepared by strong caustic potash tVom scruni- 

 allmmin, the rotation rises from -56 (that of scrnni-albuinin) 

 to -86, for yellow light. Similarly prepared from c^-albumin, 



om - 38-5 to -47, and if from coagulated whit-- of 

 it rises to-58'8. Hence the existence of various forms of alkali- 

 alliumin is probable. 



The <ulistance 'protein,' described by Mulder.- appeal-, it" it 

 at all, to be closely connected with this body. All sul>- 

 sei|iient observers have however failed to confirm his views. 

 and it is only meiit ioned here from its historical iir Since 



Mulder's time the name has been applied to various forms of 

 proteid 



"'ii-iitwn. The best method is that originally introduced by 

 Lielierkuhn. :: Purified white of e^ (see p. 1 I i i- made int.. a. 



bj^ the ad3itioji__ \vith rapid stirring of strong nqustip- ^\-.\ 

 Avoiding as far as possible all excess of ihc_jaj(' i The jelly i*- 

 ut into small Tumps and \va-hed in distilled watei. 



,/ rim n,,,,!. until the lumps are quid- white throughout. 

 The lumps of purified albumin are tli.-n di<snlved in water .JiSL 

 gentlv heating on a water-bath, the solution jjlteml and the 

 alkali-albiinijii precipitated by rarefuladdition of m-i-tic add. 

 The precipitate is then thoroughly WftsTieTl with distilled \ 



.k:i. /--. ,;t. S.'C al-. S,. \lilot.. /.. fi I'"*! .('firm N 1 U fl I-"- > 1 

 ,/. Ch. ,>. I'h.tnn. IM XVMII (1838), 8 "I 



.' 1',, --,.,,, !,, r f - .\nnitl H.I i\\\\i < II- 



