CHEMICAL I'.ASiS OF TIM: \M.\1AL UuDV. 1.'7 

 of 8 10 p.c. sodium chloride solution, {nnecipiteted from th. 



the additii ..... f ;H: ol \\ater, and purified by resolution m 



tin- salt and reprecipitation by tin- addition of water. Tin- opera - 

 tionsmust In* conducted as rapidly as possible since the pj-o- 

 loii,ued"licti(m of water renders tin- vitellin insoluble in saline 

 solutions. rTanyattempt is made t separate tin- vitellin from 

 lecithin residues by means of alcohol it is at OIK c converted into 

 ordinary coagulated p 



:;. Paraglobulin. 



This proteid IOMHN iiHjstdiaracteristically in blood-serum (al-o 

 in lymph), in amounts nowknowri to be much larger than \\a- 

 at one time Mipin^-d, and thus constituting about one-half of tlu- 

 total proteids of the .serum. - 



partition* The older methods con>i.-i.-d in flj diluting 

 serum ten-fold with water and j'assin^ a jirolon^ed current of 

 carbonic acid -as; (lj) saturatin- serum with sodium chloride. 

 The amount of precipitate thus obtained rejre>ent- only a small 

 part of the total paxaglobulin present in the scrum. 4 and the only 

 satisfactoi-y method of preparing it pure and in considerable i|iian- 

 tity is as follow- : '.'> serum is saturated at ."() with magnesium 

 sulphitj.e, liv nuuins o w'liicl| paraulobulin is tiuantitativelv |ue- 

 ripitjited The precipitate collected bv filtratign i.s_jj_istril.uU'd 

 dirou^h a small volume of a saturated solution of the magnesium 

 salt, cijllectedjon a filter and \vashed with. satiuai'<l solution of 

 M^S() 4 . JJy this means it is separated from the larger part of 

 tLe serum-albumin. 



T cir.-cl \[< tiliul alld I'olllliletr se^qilltioll fl'om t hi- lat tcl pro- 

 teid. two methods may be_udi4Jed. (a) Ti:- 1 ]! ipitatl ifl dis- 

 solved in water, then largely diluted and the para^lobulin further 

 separated out by jiassing a stlX'ajlMjiLd^- (^) '""' J'''''''!''!^! 1 ' 

 is dissolved as l)efore in water, the jiara^lohulin again salted out 

 bv M-S<),. this pn,ccs< ivpeat'-d s.-\,-ral tini''-. and the final pro- 

 diii eijjroin the nmunesiuni salt by di.^ 



1 This i- tin- Mil.-tanrr t.. hi.li Al. Srhmiilt j;:iv.- the iiaim- <>f til.riii" | 

 |. In -/,. C. A,it. . /'in/sml. .lat.ru "-''I 1 ' tl " 



i ./,. r..i M (187f) s.4i.-!. //-.< l i';'i pn-\i..ii-h 



'IMM-II ili-sri-ilM-i| iin.l.T tli- naiiH- ' .-..nun ra<i-in' l.y I'aniiin. (Vircli. 

 S. 17.) Tin- iiaim- j.aniulol.uliii is .In.- to Knlin- (/ 



NOW in-iK-rally ami ino-t a|i|ir<>|>riati-l\ known l.\ I In- latt.T i 



of nranMdobolio, as >iiL:^cstrii t>\ I|O|>|K--S" 



- llanunar-t.-ii. I'lln. l'..| \MI i I Wfrfrf, 



1--I . - 



l'ln/iol. ('hem. Vol. 1 ; 



4 Hammar-t. n. I,*-. r,t. II.'MiMii-. I'tln-:. : 



ll.i.iun. r~l, n. ... Mil \\m 'A. I 

 I'hi/siol. t'/i<m. H<l. vni. (ISH.-{), > 



|liat.- for tli.' <|iiantiiativ.' s.'jiaration loliuliiin (' 



Init Ilainniar-I.'n r. tin- ir-inT.il nirtli-l - 



