BASIS OP i IN: \MMAI. BODY. 31 



pla-ma. 1 The more important hctfl winch have thus le,-n made 

 out may IM- lirietly summarised a- i'.>lln\\>. Mu>d --plasma con- 

 t_ai_ns a uloliuliii-foreruuiier <>f myosin ( niyosino^.-n ") wTuVTi 

 resi-mltles lilirino^en in coagulating at ^'t . This pmteid i- r,,n- 

 \vrted into mvo>m on tin- occurrence oi' dotting liy tin- action of 

 a .-pecitic ferment. which is regarded a> l>einu do>dy related t... 

 if not identical with, an alluimose (see below). Tin- serum. which 

 is left in small (|uantitie.s only aftt-r the formation of tin- dot. 

 contains m-otdds winch coitL r ulat<- at 47" 



o." . (niyoLjlobuliu) 73, an albumin < : Iosdy n-<i-nillin^ scrum- 

 all.uniin. 



ii'n->iti<iti. : > (\) Finely dioj.jwd mnsde-substeiice is wa-lit-d 

 rapidly with cold water, to remove serum-alimmin and colouring 

 matters (baemOglODin , the residue is sijueexed out in linen, and 

 extiaeti-d for at l-a-t '2 4 hours with H' )>.<. solution of N'ij^'l in 

 \yhidi mvosin ia readily soluhle. Tin- extract is now liltejvTl lirst 

 through muslin and then thnniLfh jiajier: the filtrate is a more or 



vjadd and Quiescent solution of mvosin. From this the 

 III\OMII may lie prepared in a run- condition l>y allowing it- 

 solution in the ammonium salt to drop into a lar^e exei-^s oi ili-^ 

 The myt ts.ii! gradually settles out in a tloccule^nt 



^ 



ina_-<. which may lie further ]>urilied h_v resolution in a minimal 

 amount of neutral salt and reinvci]iitation by jiourin^ into an 

 excess of distilled \v;iter. '1'his jiiiritication must lie conducb-d 

 and at htw tem)>e.ratures. for myo<in is somewhat readily 

 liy the )>rolonue(l action of water and liecomes in^oluhlc 

 in saline -olutioiis. 4 (2) The tiuely dioj.j.i-d and \\ashed mu-d- 

 is divided into tw ..... jual portions: to one of the iifud- 



(deci-normal) hydrochloric acid is m /r /'////// added until a distim-t 

 acid reaction is nl.tained as shewn \<\ tropaeolin ( M ) ( see aliove. 

 p. IS). The two portions an- then intimately mixed to-.-ther. 

 allowed to -land some time, strained through muslin, tillered ami 

 the myosjn precipitated from the liltrate l'\ c'aieful nentrali-atiou 

 with veiy dilute alkali or lime-water. 



Apart from the general reactions which diaraeten-e ifijo>m a> 

 a ^lol.ulin. it is distinu'iiishcd hy the low tenii>erat ure i 

 aL yvhieh its siTline solutions constantly coagulate. ! 

 larinj ash residing on incineration, consisting ehielly of >alts of 

 lime. As already stated, it is coiiyerted into an in-oljd'le j 

 Ity the prolonged action of water, ami into syntoiiin l_>v the action 



oj_iicids. Thesi- StlltstaiK-' led to lie capable of reeon\er 



sion into ni\ p. 17). It is also stated r> that it 



1 II:illHiiirt..n,.//. / / IM 



lirinani. /j. f -, H ; iv. (1880), & 384. 



:,!! .-.ky, /j't'. : :> IM. v. (1 



\Vr>l. '/J '/. l>l tl ,snJ. r/,.m I'.. I 

 '" llallilnrt.li. lir nl }> I 1- 



