CHKMK AI. P,AM> <>1 THK ANIMAL l;l>\. J.; 



and most frequently olitaiiit'd by-product of proteid xvumi' 



It was first noticed amPisoIatetl by Meissner under tin- name of 



</-peptone. i- identical with Ilence-Jone-' pn.t.-id in tin- mine of 



:ualacia. and lias also been known under tin- name of pro- 

 peptone.' Of late years il ha> hei-n j. , ;l - i^-iirrin^ n..t 



infretiuently in grim-.- ami it is more than probable tTTat many' of 

 tin- older statements as to tin- occurrence of peptones in urine 

 and other fluids referred n-ally to the orcunvne,. of hemialbu- 

 nio--. It i- al>o >tat-il t_o_ori_2ir nonuallv i^ tin- inanuw of 

 Ijoiif-.- aiuj_iii reivhr()s])inalthnil. 4 Sis. n-ailily pe^oniseS 



by tryi'-iir\vitK the simultaniMms formation from tin- peptone of 

 much leucin ami tyrosin, hemialhunios,. x^rj-flv makes it^ ap- 

 j^earance in anv a]>precial)le <|uantitY in the final products of a 

 ijancreatie digestion. It is hest inviiare*! W the action of a small 

 iiinoiint of verv active iei>sin on a _eonsuleraMi- niass of lilirjn. 

 previously >\\rllftl up into a gelatinous mass liy the aetion~of 

 J p.c. H< 1 at 40 5 . Under the action of the ]epsin the lilirjn 

 lii|ii^: JOOD afl tin- i- 'iini]iK > te. dilute sodium carbonate is 



added until the reaction is just faintly nlU- : ilit-,,- l.y which ni'-ans 

 a bulky precipitate is obtained. This is removed by filtration ami 

 the tiltrat.- now contains a UxgB amount of heinialhuniosc and but 

 little [K-jitone, and may be utilised directly for the feestfl charac- 



' LC of the albuillOSe. 



paration of pun //////////" -dkowski). 6 Acidulate the 



filtrate de-.-ribed above strongly with acetic acid, add an 

 ('.'<~-~> ^riiis. to eai-h 100 .dium chloride, ami agitate the 



mixture until it i> saturated with salt. The hcmialbiini' 

 thus preeijiitated : it N now collected on a filter, washed with 

 saturated solution of sodium chloride. di-- 1 .l\c,l a^ain in v, 

 and reprecipitated by acetic acid and >liuin chloride. This 

 ie repeated, and the final prodad i- then di^-olved in a 

 minimal amount of water and fr ..... 1 from salt hydialy-is" h 

 may thi-n be concentrated, jm-cijutated by alcohol ;md drie.i 



sulphuric acid and then at 1 " 



^i'lllimiHui-. Th/- pure dry substance, is not 

 readih -olnhle in distilled water, but readily sohible in </</"> \ 



Bd. X1T. (1884), 8.M II.nnl.iiru" r (Dm. li) - Id \M 



1 This i-\|iri'imi inay !. coOTeotentll ' ,-''ii<-r.ill\ the chii/;i' 



tnliirril liv tin- uiii.ruMiii-*-'! f'-rin- 



-.ilkc.u.ski ii I., i hr<- v"ii llarn ' <'tO. 



Fleii ei Vii 



' Hallil.nrt..,, 



i.-tail.i IH-O '/J. f. /'< I '-I M\ (1 

 - Anli-itiui- /ur D.-ir-l.-ll pin. .!! .-h.-in. I'r.ipar.it.- \' 



: Dnriti" tli.- .li.ilx-i- S..IIIP low of albumuao* .. ei arc ulicht 



fnsihl,-. l-ir ia tin- |N-pt..m-!. 'A I'. /{>>' . IM i ' 'Not* on |. 



