CHKMICAL BASIS OF THK ANIMAL r,>DY. 71 



c hange was not necessarily due solely to tin* lid- and growth <>f 



rganisms in tin- solution, for it was found that the fern, 

 tion might be upletc in presence of an amount of carbolic 



acid wliidi is t'atal to th-- de\.-lopment of micro-organisms. 1 The 

 probable of an en/yme as a possible fa.-t >r in tin- whole 



ss which was thu- demon-nated wa- reduced to a certainty 

 by the exjH-riments of Mu-cnlu-.- Kmplovm- the thick mucous 



rh he precipitated the iniicin with al- 

 cohol, dried tin- jirei-ij.itate at a low teiujK-ratnre, extracted it 

 with water and found the extract to possess active hydndytic 

 ; a solution of urea. The proof of the existence of the 

 In a pathological mucous urine in which there i 

 OjUently no reason to suspect i. ;ice of any micro-organisms 



still Ifft (|XMI tin- (|Uc>ti.ii () f the isolation of the ir'in 



the mi. lo-oiuiani^m it-elf. When urine which \>\ exj.<,si; 

 the air has .-nt.-ivd into active alkaline f.-i iii'-ntat ion an. I. M 

 shown ly microscopic examination, is full of Torulae, is flliciently 

 vni'- <-a] table of bydrolisin.u' urea can lo precipitated 

 by alcohol from the dear filtrate. If on the other band the until- 

 tered urine he precipitated with an excess of alcohol and'th* 

 d with alcohol and dried in the air, a po\\,i 

 :iel which i- itself extraordinarily active, and yields to an 



soluble eiixyme which raj. idly <-on\erts 

 inunoiiia and carbonic acid. The rapidity of the c.mv. 

 udes the intervention of any developing or^ani-m. and that 

 lian^e is truly due to an en/ynie i- shown by the fact that it 

 ith e,|ual readiness in presence of chloroform. 8 



-oiiu' iiitt-rot tc. notice ln-r- that frmn \\hat lia> IM-.-M said 

 \\h.'>- activity tin- feriiu-Mtatinii is dm- <\ n-.t 

 tr^e tlit-ir i-n/yiiif int.. tin- Mirmmidiiin inediuin; \\ln-ii killt-.l 

 ic.ili.J. tln-y yii-ld it n-ailil- italile 



Thi> li..l<ls p.<l aU> in tin- case of invcrtin. \\hidi : 

 found in tin- liltrat.- !>..; \\liili- it ina\ n-adily IM- .-\t ra.-t. : 



tin- .-.-II- when killed l-y ether .T al.-..||,d. 4 Similarly it appear- that 



\ield an en/ynie ho>e acti-m i> 

 ryp-in. 



The nio-t j.rolit: of the urea enzyme is in all case- the 



mucous urine passed in inflammatory conditions of the bladder. 



II:- i U 



I i \\MII 1 1 -:.). i - I.\\M 



<mpt. Rtnd 1 i \\MII j. 

 1 Le. 



4 Mopp^Seyler. !:< <l. A 

 ' 



i Kjo|,|hl(I>.. Al-t in 



Indu*!. IH^I.N.K. |. .'. '*" 



* I! 

 l>1ni*inl. Cktm. Bd. XI. (IWT K . Salkuwnki. /J ' H>-J Bd xx\ (IBM). 



