CIIK.MICAL BASIS OF THK ANIMAL IHIV. L M7 



and becomes ordinary ha-matin : it is in fact the substance usually 

 spoken of as reduced ha-matin. (See I..-1..-.. 



L'. Oxy-haemoglobin. When luemo^lobiu is exposed ti> tin- 

 air it rapidly unites. molecule^ tor molecule, \vitli oxygen, thus 

 becoming oxy-lui-iiKixl' >bin, the characteristic constituent of the 

 red-corpuscles to which the scarlet colour of arterial b|u<>d is du-.' 

 readily set free from the corpuscles b\ i h- addition to 

 defibrinated Mood of such rluids as alcohol, ether, chloroform. 

 wa_ter, and solutions of bile-salts. or^jbj_jx'jji^edl^fivexiii" "and 

 thawing the blood : when, thus set free it passes 7 into soIutiou"iM 

 the jidjaeent serum. Froiu this solution it may ! obtained a- 

 Aith more or less readiness. dependency upon the kind 

 of animal whose lilood is n-.-d f..r its preparation see vj ."-U, 1 , the 

 ditl'crciicc l.riim due. j-arily at least, to tlu- varying soluliility of 

 tin- several haemoglobins. 



.iitain rapidly a microscopic preparation of o\y-ha-nio^lol.in 

 Is it >utli< -es t< take a drop of the blood of some animal 

 WOOee ha-nioulol.in ery>talli-e- r.-adily (rat, guinea-]ii^, or d- 

 mix a drop of it on a slide with a minute drop of water, and allow 

 the mixture to evaporate until a rin^ of dried substance is formed 

 at the periphery. It' it lie now cnveivd with a rover-slip, ery-taN 

 usually form in a short time, especially if it he kept cooled. I : 

 the preparation of axy-hflemoglobin crystals IIM ; i ]aii, r e scale many 



methods, the >ame in general principles hut diilerin^ >om ( -what 

 in detail, have lieen proposed, the ditlieulty of the ju-eparatiou 

 varyin.u' cousideialily acconlinu to the kind of him id u>ed.- 

 laJratory ]>urpo<es lar^e ((iiantities of crystallised o\y-ha-nn>- 

 ^'lohin may he \. i\ readily ohtained from do^'s hloi.d ;>> follows 

 Kiihue). 'JJn^ blood is detihrinated and strained tlirouyh tine 

 muslin : it is then placed Fn a llask and etlier is julded with 

 frequent shaking until the 1-lood i- ju>t lak\ .n-] ai.-nt. 



The tlask is imw -iiiiMiuided 1. in^ mixture of ice ;ind -alt 



and in a short time it- content- u-ually liec.,me almo-t ; 

 from the m;i- rVStalfl which form in it. Ti. then 



centrifu^'ali-ed oil', dk-"l\ed in a minim"! amount of 



...1,-d ! It . and after the additio: ! its 



hulk d alcohol a^ain imnier-.-d in ;i flee/in^' mixture. 



The --Miid crop . |fl thu> ohtained may he a^ain \< 



talli-ed U aln-.nly d- I '-hed 



with water at >' r-ontainin-j -' p '' "t alcnhol. and may he diie.l 

 -ulphurie acid at O'.and an- now fairly stable. 



.>\>\\\ is uniti-.l ^ in tin- lil.l .( all I, wiili i. 



In in\Tti-l>r:iii- i,l,. ; illy fmui'l in f.>luti.m in the pliwma, 



I. ut tli.-r<- :in- :i few (riirht iN :i"'l litermture Me 



I!;illilnirt..n. ''I,. ! <in<l I'.it 



r fnll-r -i 



l>ln/.<,ol. ' 



- 18 I! !. /'AyWo/. / 'ctttchr. f. C I 



,1. T. 109 (1890), }. 



