BACTERIOLYSINS AND IL-EMOLYSINS 89 



If to a hsemolytic serum derived from guinea pigs 

 we add an anticomplement serum derived, as just 

 stated, from rabbits, and containing an anticom- 



* I 



ii. 



f 



COMPLEMENT 



COMPLEMENT 



ANTICOMPLEMENT 



IMMUNE BODY J| f IMMUNEBODY 



CELL MB m m CELL 



FIG. 8. (After Levaditi.) 



plement specific for guinea-pig complement, the 

 haemolytic action of the former will be inhibited, for 

 the reason that the complement necessary for the 

 haemolysis to take place has been bound by the 

 anticomplement. (See Fig. 8.) One must, how- 

 ever, observe the precaution to heat the anticom- 

 plement serum of the rabbit to 55C. before so 

 mixing it, in order to destroy the complement which 

 it contains and which would otherwise reactivate the 

 guinea-pig immune body. 



From the foregoing we see that either anti- 

 immune body alone, or anticomplement alone, is 

 able to inhibit the hxmolytic action. Haemoly- 

 sis cannot take place when either of the two 



