Step 3. Connect a 2-foot long x 14-inch O.D. 

 polypropylene tube (B) between the front left 

 connection of the valve cabinet and the auto- 

 matic sampling valve, using a Perkin-Elmer 

 fitting at the valve. 



Step 4. Connect the automatic sampling 

 valve and the bottom of the glass sampling 

 chamber with a 6-inch long x %-inch O.D. ny- 

 lon tube using a nylon fitting on the glass sam- 

 pling chamber and a Perkin-Elmer fitting on 

 the valve. 



Step 6. Connect a 2-foot long x i^-inch O.D. 

 tube (H) between the 14-inch O.D. tube from 

 the partitioner and the rear right connection of 

 the valve cabinet. 



Step 6. Connect a 2-foot long x 14-inch O.D 

 Ascarite Drying tube (I) between the %-inch 

 O.D. tube from the partitioner and the front 

 right connection of the valve cabinet. 



3. The following instructions apply to both 

 systems : 



Step 1. Insert a silicone rubber serum cap in 

 the top of the glass sampling chamber. 



Step 2. Connect one end of a 1-foot long x %- 

 inch O.D. nylon tube (D) to the bottom side 

 arm of the sampling chamber. Use a nylon fit- 

 ting. Connect the other end of tube (D) to a 

 toggle valve, and run a tube from the toggle 

 valve to a liquid waste container. 



Step 3. Connect a 2-foot long x %-inch O.D. 

 nylon tube (F) from the automatic sampling 

 valve. Connect a metal fitting on this tube and 

 connect a rubber bulb to the fitting. 



Step i. Connect a 2-foot long x i/4-inch O.D. 

 nylon tube (G) to the automatic sampling 

 valve. This tube goes to the sea water sample. 



Step 5. Connect the two-stage regulator (J) 

 and the line regulator (K) to the 200-cubic 

 foot helium cylinder, lashing the helium cyl- 

 inder (fig. J-8) securely to the bulkhead to pre- 

 vent its toppling over ni rougli seas. 



Step 6. Connect a 2-foot long x 14-inch O.D. 

 Driente filled drying tube (L) to the line regu- 

 lator, and connect a 6-foot long x %-inch O.D. 

 polypropylene tube (M) between tube (L) and 

 the "in" connection on the gas partitioner. 



Step 7. Connect one end of a 2-foot long x 

 3/8-inch O.D. tygon tube (N) (figs. J-10 and 

 J-11 ) to the "out" fitting of the gas partitioner. 

 Connect the other end of tube (N) to the side 

 arm (O) of the bubble counter 25 ml. burette. 

 Place a rubber bulb (P) filled with a detergent 

 solution on the bottom of the burette. Mount 

 this flow meter near the sampling valve with a 

 burette clamp (fig. J-8) . 



Step 8. Connect the shielded cable to the out- 

 put terminals of the partitioner and the input 

 terminals of the strip-chart recorder (fig. J-12) . 

 Connect the three black binding posts on the 

 recorder with a strip of solder or a wire. 



Step 9. Connect power cables of the parti- 

 tioner and the recorder to a 110-volt outlet, and 



J-16 



turn on the partitioner thermal stabilizer heater 

 toggle switch. It will require about 8 hours to 

 stabilize the temperature of the partitioner 

 above laboratory temperature. Ccmtion: Do not 

 turn on the cell power switch until helium is 

 flowing. 



Step 10. Open the two stage regulator on the 

 helium cylinder until the gage reads 30 psi, 

 and adjust the line regulator valve imtil the 

 gage reads 12 psi. Set the four- way valve to By 

 Pass position, and check all connections with a 

 leak detector. A detergent can be used to im- 

 provise a leak detector. 



^ 



5 



Figure J-12. Two-conductor cable connections be- 

 tween gas partitioner and recorder. 



J-21 How the System Works.— The flow path 

 of the carrier gas through the gas chromato- 

 graphic system is controlled by the four-way 

 valve and the automatic sampling valve. Fig- 

 ure J-13 shows the flow path of the carrier gas 

 through the system. 



1. With the four- way valve in chamber posi- 

 tion, the gas flow is measured and adjusted, and 

 the partitioner and recorder are calibrated. 



2. With the automatic sampling (eight-way) 

 valve in the Syphon (clockwise) position, a 

 precise amount of water sample is syphoned into 

 the system. 



3. When the gas flow, partitioner, and re- 

 corder are adjusted and the sample has been 

 syphoned into the system, the eight-way valve 

 is turned counterclockwise to {position Fill 

 Chamber and the four-way valve is turned to 

 the Chamber position. This forces the water 

 sample into the sample chamber where the he- 

 lium bubbles through the sample and strips it 

 of its dissolved gases. 



4. The He gas and the stripped dissolved 

 gases flow through the partitioner drying col- 

 umn and then through the Ascarite which ab- 

 sorbs the CO2. The 1st detector then measures 

 the thermal conductivity of the remaining com- 

 posite (He, Nj, O2, and A) gas. 



5. The composite gas then flows through the 

 gas partitioner molecular sieve column which 

 separates the Nj gas from the O2-A gas. 



6. Thus, the strip-chart recorder, which is ad- 

 justed to zero for pure He, records three peaks: 

 Peak 1, the composite gas; Peak 2, the O2-A 



