June, 1921. 



SCIENTIFIC AGRICULTURE 



259 



Table /.—Development of cowpea-soybean bacteria, Bacillus radicicola 

 (from clover, vetch, etc.), and B. radiobacter 



Substrates. 



Cowpea-soybean bacteria. 



Mannite-nitrate 

 agar slant 



Macroscopic examination. — Raised 

 whitish to porcelain white, glossy 

 layer. 



Microscopic examination. — After 3 

 days slender rods, sometimes curv 

 ed: after 7 to 10 days unstained 

 irregular sheaths, with 1 to 4, most 

 frequently 2, darkly stained granu- 

 les: after 2 to .3 weeks many small 

 globules, ovals, and short rods out 

 side of the unstained sheaths also 

 small globular regenerative bodies 



Beef agar slant. | 



Beef gelatin 

 stab 



Beef broth. 



Milk. 



Potato 



Macroscopic examination. — Fairly 

 good whitish growth. 



Microscopic examination. — After .3 

 days weakly stained, irregular, 

 thin, short rods; after 7 to 10 davi- 

 irregular rods, producing gonidia 

 and globular regenerative bodies, 

 which may multiply as such; after 2 

 to 3 weeks very variable apoearance, 

 rather long slender rods, often 

 branched, or club-shaped, glo- 

 bular regenerative bodies, also 

 unstained, irregular sheaths with 

 dark granules, and large globular 

 gonidangia. 



B. radicicola (from clover, vetch, etc.) 



B. ladiobacler. 



Macroscopic examination. — Slimy, 

 transparent growth, with or without 

 whitish streaks. 



Microscopic examination. — Small 

 ovals and short rods, producing after 

 1 to 2 weeks gonidia and small globu 

 lar regenerative bodies. Also un- 

 stained slime threads with dark 

 granules and large globular, or oval 

 gonidangia; irregular pale forms 

 from symplasm. 



Macroscopic examination.— Thick, 

 slimy transparent layer, with whitish 

 streaks. 



Microscopic examination. — After 7 

 days small ovals and short rods, im- 

 bedded in slime; after 14 days some 

 rods with thick unstained capsules 

 forming symplasm; after 3 to 4 weeks 

 normal cells, stars, and large globules 

 and clubs from symplasm. 



Macroscopic examination. — Meager, 

 flat, grayish growth. 



MiCROScopic ex.'kmination. — Mosth' 

 small ovals and short rods, the latter 

 sometimes curved, budding and 

 branching; later also large rods, and 

 large globular, oval, or club-shaped 

 gonidangia. 



Macroscopic examination.— Very 

 small, gray, nonliquefying disk or 

 the surface, hardly any growth in 

 the stab. 



Microscopic examination. — Thir 

 rods, sometimes branched or swoU 

 en, producing gonidia and smal glo 

 bular regenerative bodies; in old cul 

 lures gonidia and regenerative bo- 

 dies frequently predominating. 



Macroscopic examination. — Broth 

 at first clear, with little sediment ■ 

 later (after about 2 weeks) slightly 

 turbid. 



Microscopic examination. — After 

 3 days slender rods, sometimes cur- 

 ved; after 2 weeks granular rods 

 producing gonidia. also budding and 

 branching, small globular regenera- 

 tive bodies, and symplasm; after 3 

 to 4 weeks very irregular forms, 

 branching, swelling. 



Macroscopic examination. — During 

 the first weeks no change visible, 

 later slow digestion, no clear serum 

 zone. 



Microscopic examination. — Mostly 

 small globules and ovals, few short, 

 slender rods. 



Macroscopic examination. — Very 

 meager, transparent, thin layer. 



Microscopic examination.— After 7 

 days slender rods, sometimes'branch 

 ed.'or'with'terminal'swelling; after 

 4 weeks'small globules and ovals 

 irregular rods (frequently branched), 

 globular regenerative bodies, and 

 symplasm with vcr\' variable new 

 development. 



Macroscopic examination. — Small 

 gray nonliquefying disk on surface, 

 very little growth in stab. 



Microscopic examination. — Small 

 ovals and short rods, gonidia. and 

 small globular regenerative bodies 



Macroscopic examination. — Broth 

 either clear or ver>' slightly turbid 

 little whitish sediment. 



Microscopic examination. — Small 

 ovals and short rods, budding and 

 branching, occasionally threads; aft- 

 er 1 to 2 weeks many gonidia and 

 small, globular regenerative bodief 



Macroscopic examination. — After ' 

 to 4 weeks clear serum zone on top. ".' 

 to ."■) mm. thick: milk below nearly 

 unchanged, very tine flocculation. 



Microscopic examination. — Small 

 ovals and rods, later also granular 

 threads and symplasm. 



Macroscopic examination. — Meager, 

 transparent, slimy growth. 



Microscopic examination. — Small 

 slender rods, budding and branching, 

 some ovals, globular Fregenerativt 

 bodies; later gonidia predominant 



Macroscopic examination.— Flat, 

 whitish slimy layer, thick sediment 

 below. 



Microscopic examination. — As on 

 mannite-nitrate agar. 



Macroscopic EXAMiNATioN.-Grayish, 

 flat, round, nonliquefying surface 

 growth, little growth in stab; after 2 

 to 4 weeks gelatine sometimes brown 

 on top. 



Microscopic examination.— Typical 

 ovalsand shortrixis. these sometimes 

 cur\'ed or branched, also unstained 

 slime threads wUh dark granules, 

 later symplasm with stars^ 



Macroscopic examination.— Broth 

 turbid, white ring, whitish rilm,much 

 whitish sediment. 



Microscopic examination.— Small 

 ovals and short rods, budding and 

 branching: later gonidia, globular 

 regenerative bodies, threads, and 

 fine stars from symplasm. 



Macroscopic examination. — First 

 slime ring and serum zone on top 

 later whole milk turning brown. 



Microscopic examination.— After 7 

 days typical ovals and rods; later 

 small and large cells from symplasm . 



Macroscopic examination.— First 

 gray later coli-brown slimy layer, 

 potato turns frequently gray. 



Microscopic examination. — First 

 small ovals and short rods, budding 

 and branching, later also large oval 

 and globular gonidangia and sym- 

 plasm with stars. 



