Moore. — Study of Trout Diseases. 91 



were placed in the Cold Spring hatchery, on Long Island, some in the 

 hatchery at Caledonia, N. Y. and others were presented to the U. S. 

 Bureau of Fisheries, being transferred to Mr. Clark at their Michigan 

 station. Scanning the literature for records of outbreaks of disease 

 both previous and subsequent to the importation of the eggs, I have 

 found very little authentic data to support my theory. Nothing but a 

 devastating epidemic has seemed worth mentioning in the annual re- 

 ports and these have not always been accompanied by the attending 

 symptoms of the disease. There must have been in the nature of things 

 many lesser epidemics. In this connection a too great modesty has 

 prevailed in mentioning them, perhaps for statistical reasons (laughter) , 

 consequently we are deprived of much valuable information that might 

 serve as a basis in arriving at some conclusion as to the effects of these 

 foreign importations of fish eggs. 



Mr. Leach: This has been a very interesting paper, because the 

 subject is one of vital importance to everybody interested in trout. I 

 want to give all an opportu.nity to discuss the matter with Dr. Moore, 

 and, if she is willing to continue, we shall be glad to hear from any 

 others who wish to ask questions or speak on the subject further. 



Mr. Titcomb: If you require .specimens for examination, are fresh 

 specimens necessary? 



Dr. Moore: It is possible to use preserved specimens, but generally 

 the preservative used is the one that is handy and that one is not usually 

 the most desirable for further study of the organism. The most ideal 

 way is to visit the hatchery with a microscope, then a diagnosis is 

 possible, often within a few hours. Fresh specimens in sufficient num- 

 bers are the next best thing. 



Mr. Titcomb: For the benefit of the laymen in the fish hatcheries 

 absent from this meeting, will you tell us what is the best way of putting 

 up fresh specimens to be shipped for examination? 



Dr. Moore : Shipment in cans alive by ordinary shipping methods is 

 the best way. Diagnosis is sirapMfied and in some cases only possible 

 when the fish are sent before death ensues, for the reason that various 

 invading organisms following death, such as bacteria, protozoa and the 

 like, may obscure entirely the correct line of diagnosis. When fish 

 cannot be shipped in cans I have found it useful to have them wrapped 

 separately in a moist cloth, packed in ice, and expressed with dispatch. 



Mr. Bullock: Would shipment in a solution of formaldehyde an- 

 swer the purpose? 



Dr. Moore: Formaldehyde is a useful preservative, but it is used 

 indifferently at various concentrations. I do not know, however, of 

 anything better for general purposes. 



Mr. Doze: The brown trout have been introduced in the higher 

 altitudes. Have you ever received specimens from the Rockies and do 

 you know whether or not this disease is prevalent there? 



Dr. Moore: I have not received specimens from that quarter. I 

 think it would be vei'y desirable to get this information and for the 



