American Fisheries Society. 123 
The loss during the year among the first lot amounted to 14 
females and 16 males, while that among the second lot was 8 
females and 7 males, or exactly half the loss in the first pond. 
The fish in both inclosures began spawning October 3. Of the 
first lot 75 females yielded an average of 1021 eggs or 31 less 
than the average of the previous year; 89 males were used for 
fertilizing them. ‘Two per cent of the eggs were poor when taken 
and a further loss of 7 per cent occurred before the date of 
hatching. The average weight of these fish when placed in the 
pond was 7-8 of a pound, and at the spawning period it showed 
no increase. Their color also was somewhat dulled. 
The yield of eggs from the second lot of fish showed an 
average gain of 18 eggs per fish. ‘To impregnate this lot 86 
males were stripped. One per cent of the eggs were poor when 
taken and a further loss of 4 per cent ensued during the hatch- 
ing period. The fish had gained an average of an eighth of a 
pound during the year and their colors were brighter than when 
placed in the pond. 
The fish of the second lot produced an average of 49 more 
egos per fish than the first lot, and the loss up to the time of 
hatching was four per cent less. The loss of fish by disease in 
the first lot also exceeded that of the second lot by 15, but there 
appeared to be little, if any, difference in the fry hatched from 
the two lots of eggs. 
Nearly all of the fish lost suffered from gill affection. Both 
ponds during the course of the experiments were kept scrupu- 
lously clean, and so far as it was possible the conditions in both 
were made identical. 
