American Fisheries Soctety. 103 
EYED WHITEFISH EGGS. 
“Whitefish eggs nearly ready to hatch have a much lower 
specific gravity than lake trout or brook trout eggs. At 1.034 
nearly all float, while at 1.029 they sink immediately. The best 
separation occurs at about 1.029. Eggs smothered or acid killed 
on the previous day may be almost completely separated by the 
above solution, although such eggs do not turn completely white 
within 24 hours after death. Like the lake trout, miscellaneous 
dead eggs dying naturally but not the same day, in the jars, 
do not separate completely, but yet a large percentage may be 
removed. 
RAINBOW TROUT EGGS. 
“Living eyed rainbow trout eggs from Wytheville, Virginia, 
nearly ready to hatch float at 1.079 and sink immediately at 
1.060. At 1.066 considerable separation occurs, but the most 
favorable strength is about 1.072. Acid killed eggs may be 
thoroughly separated, though not perfectly, by this strength. 
“The eggs for these trials were killed by pouring a little 
dilute hydrochloric acid upon some eggs in water in a beaker. 
Great activity was immediately shown by the embryos, which 
soon ceased (within 144 minute), leaving the embryos faintly 
whitish so that the eggs were distinguishable immediately from 
good eggs. After washing away the acid and returning the eggs 
to the tray on the water flow, the embryo after about one hour be- 
came more conspicuously white, and the next day the whole egg 
had turned white. 
“Some unfertilized rainbow trout eggs from Manchester, 
Towa, accompanying a shipment of eyed rainbow eggs, were tried 
in various salt solutions with a view both of separating them 
from other eggs and of determining the effect of the salt solu- 
tion after returning the eggs from it to water. 
“No separation of the unfertilized eggs from living eyed 
eggs could be made, as they were found to float or sink at prac- 
tically the same time. They can, however, be separated from 
dead eggs turned white in the same way that the fertilized eges 
are separated. 
“The unfertilized eggs failed to turn white after treatment 
with the salt solution and return to fresh water. Following Mr. 
