104 Thirty-fifth Annual Meeting 
O’Malley’s procedure, a 1 to 20 solution was made, which had a 
specific gravity of 1.039. The unfertilized eggs were immersed 
in this, allowed to remain for a half-minute, and then returned 
to fresh water. Even after remaining in water over night they 
had not turned white, and were not distinguishable from untreat- 
ed eggs. Stronger salt solutions were tried, but even after 1.125 
for 25 minutes they did not subsequently change their appear- 
ance. 
“The most effective separation of white eggs from live eggs 
and from unfertilized eggs which had not turned white was in 4 
solution of specific gravity 1.077, and not in 1.072 as with former 
lots. 
BROOK TROUT EGGS. 
“Living eyed eggs nearly ready to hatch float in 1.077 and 
sink in 1.072. Specific gravity 1.0725 separates acid killed eggs 
almost perfectly, nearly every dead egg rising and nearly every 
live egg sinking within the first minute. There was no oppor- 
tunity to try eggs which had died in the trays. 
REMARKS. 
“These trials refer only to eyed eggs and unfertilized eggs 
of the same age. Green eggs have not been tried, but since it is 
claimed that salt solution has much separating power with un- 
fertilized eggs (salmon) at an early stage of development” and 
with “very immature eggs”, it may be conjectured that the same 
will hold true of trout eggs. 
“Hyed eggs which have died simultaneously may be well 
separated by this method. Eggs which have died successively 
vary in specific gravity and can be separated less completely, but 
nevertheless considerably. Its application practically will de- 
pend mainly on the condition of the dead eggs in this respect, 
but is indicated as possible with each species named and probable 
with the brook, rainbow, and lake trouts. 
“The salt solution should always be adjusted with a hydro- 
meter (salinometer). Special salinometers may be made to 
order to some advantage, but the ordinary hydrometers on the 
market will answer. Salinometers made for sea water are in- 
applicable for any of these species save the white fish, since they 
