223 On Budding in Perophora. 
In fig. 5 are shown two sections of the tube and ganglion 
taken from the same bud, A being more anterior than B. 
The series of sections from which these are taken shows a 
eradual transition from the one to the other, and establishes 
Fig. 5. 
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29/0 
A B 
in the clearest way possible that the ganglion is pinched off 
from the thickened ventral wall of the tube, the constriction 
beginning posteriorly and gradually proceeding towards the 
anteriorend. This fact I have verified not only on B. Gouldit, 
but also on an undetermined species of Bodryllus obtained in 
Jamaica. 
EXPLANATION OF THE FIGURES. 
EC. Ectoderm. PC. Pericardium. 
END. Endostyle. PH, Pharynx. 
EN. V. Endodermal vesicle. PT. Double-walled partition of stolon. 
INT. Intestine. ST. Stolon. 
L. PBC. Left peribranchial sac. LT. Test. 
k. PBC. Right peribranchial sac. 
All the figures are drawn with the camera. 
Figs. 1, 2, and 3 are transverse sections of the posterior region of buds 
of Perophora viridis, Verrill. Fig. 5 is taken from a specimen of the 
Jamaica species of Botryllus. 
Fig. 1.—Very young bud. Shows connexion with the stolon, rudiment 
of the pericardium, and thickened wall of inner vesicle. This 
figure differs from figs. 2 and 3 in that its right side is the left 
side of the latter, and vice versa ; in tig. 1 the face of the sec- 
tion is towards the anterior end of the bud, whereas in figs. 2 
and 3 it is towards the posterior end. 
Fig. 2.—Somewhat older bud. Rotation of the inner vesicle has pro- 
ceeded to some extent. Formation of left peribranchial sac 
is beginning, 
Fig. 3.—Still older bud. Rotation is nearly completed; right peri- 
branchial sac is present. 
Fig. 4.—Shows three stages in development of rudiment of nerve-ganglion 
and hypophysis in Perophora viridis, V. 
Fig. 5.—Transverse sections of dorsal tube and ganglion of a Botryllus 
bud, showing constriction of ganglion from thickened ventral 
wall of the tube. A is more anterior than B. 
