452 



BULLETIN OF THE BUREAU OF FISHERIES. 



Table XIII. — Depression of Ihefreenng point produced by the blood, the serum, and the ovarianfluid from 



the same animal. 



Ann. -'! 

 Aug. 24 . 

 Aug. 28 . 



1901. 



Aug. 20 . 

 VilL'. 23. 

 Aug. 29 . 



No. 



207 

 211 

 225 



Mean lor 6. 



° a 



A =-0.586 



5S3 



-0. 628 



-0. 576 

 -0.614 

 -0. 638 



Serum. 



°C. 

 A = -0.590 

 -0. 588 

 -0.625 



-0.579 

 -0.596 

 -0. 635 



Ovarian fluid. 



° C. 

 \. = -0/538 

 -0. 552 

 -0. 546 



-0.559 

 -0.548 

 -0. 571 



Table XIV. — Depression of the freezing point produced by the strum and by the ovarian fluid from the 



same fish. 



These tables, together with Tallies XI and XII, demonstrate that the ovarian 

 fluid has an average concentration less than that of the blood or .serum of from 

 0.060- C. to o.osii C. The ovarian fluid in those fish in which the serum also was 

 measured has a mean or average depression of the freezing point less than that of 

 the serum by o.o.W (J. In the six instances in which the whole blood and the serum 

 also were determined the difference between the .serum and ovarian liquid is 0.054° 

 ('.; between the whole blood and ovarian fluid, 0.056 c C. 



Considering all the determinations on female salmon the mean difference becomes 

 somewhat more, viz.. 0.05t! : and 0.061 c C, respectively. (See Tables XI and XII.) 

 On the evidence at hand it would appear that the ovarian liquid bears a constant 

 difference in concentration, represented by a difference in the freezing point, of 

 from 0.050 c to 0.060 c ('. less than that of the blood plasma of the same animal. 



The origin of the ovarian fluid in the salmon needs further investigation. In 

 the one unripe female which I used in experiments, in which the eggs were not yet 

 set free in the body cavity, the eggs were free from excess moisture and there was 

 no appreciable amount of liquid in the abdominal cavity. Mr. G. H. Lambson, the 

 superintendent of Baird Station, writes me in response to my inquiry: 



We have opened many females before the eggs were ripe, but never have noticed any egg fluid in 

 them. The eggs before they separate are rather dry and do not wet the fingers^ When we open the 

 green females it is to secure the eggs for fishing and we may have overlooked the fluid, but from the 

 fact that the eggs are practically dry I should think none was present. 



