270 IOWA ACADEMY OF SCIENCES. 



In regard to the last plates poured it is a singular fact 

 that but a very small development occurred, and this is 

 strange since we had such an unusual development before 

 running from 6,000 to 18,000 per cubic centimeter. 



In regard to the condition of the well it looks as though 

 the water coald easily have drained off from the surface, 

 but nevertheless upon removing some of the boards from 

 the top of the well I found that the water might easily 

 have entered between the cracks of some of the boards. 

 In fact I found moisture on the inside on the upper tile, 

 showing the water had run down. One can readily see 

 how B. coli-couuuiinis or other foreign organisms could get 

 into the w^ater. Gas was produced in one tube poured by 

 Mr. Faurot and a slight amount in another. In this case 

 we made the usual test. We also obtained gas from the 

 first plates that I poured. 



The samples collected on October 29 were kept for forty 

 days in the laboratory and then were examined by Mr. 

 McKinley and Mr. Thomas with the following results: 



Various species were found. Some of these have been 

 excluded as having no connection with Bacillus ft/pJiosus 

 or B. coU-communis. On the other hand there are a number 

 of species that belong to the typhosus group culturally so 

 far as has been carried out. Our work was interrupted 

 although cultures of all of the species were made and placed 

 away for further study. Fire destroyed the entire labora- 

 tory so no further study can be made. 



One peculiar pearly white Bacillus developed in consid- 

 erable quantity, in fact at least three-fourths of the colon- 



