PROCEEDINGS OF THE THIRD ENTOMOLOGICAL MEETING 



The Pasteur system of examination is the best for elimination of 

 pebrine in univoltine, bivoltine and multivoltine races in India. The 

 system is more simple, more economical, and less laborious than all the 

 existing modified methods of Pasteur. For easy and Cjuick detection 

 of pebrine corpuscles from the juice of mother- moths the following 

 precautions should be adopted : — * 



(1) Place 50 or 60 mother-moths on a paper after separating the 



pairs and shake the paper by holding one of its ends. Tie 

 moths will excrete the contents of the colon so that there 

 will not be much exudation of the colon at the time of crushing 

 them for microscopical examination. 



(2) Keep the mother-moths isolated in paper bags on the first 



night after oviposition at about 80°-95°F. After t or 5 

 days dry them for three or four hours at about 160°-180°F. 

 The number of the bag containing a moth should correspond 

 with the number of the laying oviposited by it so that the 

 eggs laid by each moth can be ascertained. This process 

 will help the multiplication of pebrine corpuscles and dry up 

 the contents of the colon. 



* In Japan multivoltine moths are e.xamined with or 

 countries tliey are examined without drying. 



■ithout drying. In somi; 



